MB Molecular Biology (ASCP) Practice Exam Updated Q&A for 100% Pass 2025/2026

ASCP MB • Wed May 28 2025

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Get access to the latest Molecular Biology (ASCP) Practice Exam with real-style questions and verified answers. This comprehensive Q&A guide is tailored for 2025/2026 candidates preparing for the MB (ASCP) Board Certification Exam.

What is the MB (ASCP) Exam?

The MB (ASCP) certification is designed for molecular biology professionals seeking recognition from the American Society for Clinical Pathology. It's one of the most respected credentials in the field and is widely accepted by employers and labs across the U.S.

Whether you're a medical laboratory scientist, molecular technologist, or biotech student, passing this exam boosts your career and credibility.

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🎯 Why Use This MB ASCP Practice Exam Q&A?

This MB practice exam is not just a question dump. It's carefully structured guide to simulates the real ASCP exam format; based on past papers, and covers the key domains in detail:

✅ 100+ Practice Questions
✅ Detailed Explanations for Every Answer
✅ Updated for the 2025/2026 Exam Blueprint
✅ Instant PDF Download
✅ Covers All Major Content Areas

Here are some Sample Questions and the Correct Answers:

If a woman is infected with the HPV virus, which of the following parameters increases her risk of developing cervical cancer? - Answer - Smoking

All of the following are components of nucleic acids, EXCEPT:

- Phosphate group

- Sugar (ribose or deoxyribose)

- Nitrogenous base (A, C, T, G)

- Formamide - Answer -Formamide

Imagine you are working in specimen processing for the molecular laboratory and you receive a patient's specimen collected in a lavender top vacutainer tube containing EDTA. The test that is ordered requires that the specimen be collected in a yellow top ACD vacutainer tube. What is the BEST action to take in this instance? - Answer -Follow the prescribed laboratory protocol for accepting and rejecting specimens

Which of the following is not a component of a nucleotide?

- Phosphate group

- Anti-codon

- Ribose sugar

- Nitrogen base - Answer -Anti-codon

The nitrogenous base in a nucleotide is expected to be found on which position of the sugar? - Answer -C1'

What genes would be screened in a lung cancer panel? - Answer -KRAS, EGFR, ALK

What does the term consanguinity refer to? - Answer -Individuals that share a common ancestor or are considered "of the same blood"

What is multiplex PCR? - Answer -A PCR technique to amplify multiple targets

When this enzyme finds nicks or gaps in the sugar-phosphate backbone of DNA, it closes them! - Answer -Ligase

What assay amplifies the probe signal and the probe RNA concentration increases if the target to be detected is present? - Answer -Qβ replicase

What is the sequence recognized by poly (A) polymerase? - Answer - AAUAAA

What type of restriction enzyme has methylation activity? - Answer - Type 1

A parent has an autosomal recessive disorder. What percent chance does this parent have to pass down this affected gene to his/her child? - Answer -100%

Splicing is the process that does which of the following? - Answer - Remove introns and preserve exons

Which of the following is not a characteristic of eukaryotic DNA transcription? - Answer -Strict promoter sequences

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